| Assay Method Information | |
| | SDFQ HTS Assay |
| Description: | SDFQ HTS primary assay using pAB1_FL905 was performed in 2 µL of (1 xDNA gyrase buffer: 20 mM Tris-Acetate pH 7.9, 50 mM KAc, 10 mM MgCl2, 2 mM DTT, 1 mM ATP, 0.1 mg/mL BSA). The following is the procedure: 1) Using the BioRAPTR, dispensed 1 µL of E. coli DNA Gyrase (350 ng/ µL) with a final conc. in assay 175 ng/µl. 2) Using the BioRAPTR, dispensed 1 µL of DNA pAB1_FL905 (6.425 ng/µL) - final conc in assay is 3.2125 ng/µL in assay. 3) Spun plate at 800 rpm for 30 seconds. 4) Incubated the plate at 37° C. for 2 hours in the dark and read the plate on the Envision measuring fluorescence (excitation@484 nm, emission@Em520). SDFQ HTS secondary assay using pAB1 _FL924 was performed in 2 µL of (1 ×DNA gyrase buffer: 20 mM Tris-Acetate pH 7.9, 50 mM KAc, 10 mM MgCl2, 2 mM DTT, 1 mM ATP, 0.1 mg/mL BSA). The following is the procedure: 1) Using the BioRAPTR, dispensed 1 µL of E. coli DNA Gyrase (350 ng/ µL) with a final conc. in assay 175 ng/µl. 2) Using the BioRAPTR, dispensed 1 µL of DNA pAB1_FL924 (6.425 ng/µL) - final conc in assay is 3.2125 ng/µL in assay. 3) Spun plate at 800 rpm for 30 seconds. 4) Incubated the plate at 37° C. for 2 hours in the dark and read the plate on the Envision measuring fluorescence (excitation@531 nm, emission@Em595). |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |