| Assay Method Information | |
| | Inhibition of Sox18-DNA Binding Assay |
| Description: | Different concentration ranges were tested in the FP-based DNA binding competition assay (0.2-200 uM, 10-500 uM, 10 uM-3 mM), with DMSO concentrations ranging from 0 to 3.33% v/v. Experiments were performed in three independent replicates. The second column summarizes threshold concentrations at which compounds Sm1-14 start forming micelles in saline (200 mM NaCl) or fluorescence polarization buffer (30 mM HEPES pH 7.5, 100 mM KCl, 40 mM NaCl, 10 mM NH4OAc, 10 mM Guanidinium, 2 mM MgCl2, 0.5 mM EDTA, 0.01% NP-40). The third and fourth columns summarise 50% inhibitory concentration -IC50- of cell-based luciferase SOX18-dependent transactivation as well as cytotoxicity -CC50- for all active compounds, in COS7 fibroblasts. |
| Affinity data for this assay | |
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