Assay Method Information | |
| Protease Inhibition Assay |
Description: | HIV-1 protease activity was measured by a continuous fluorometric assay. The proteolytic activity can be monitored by the increase in fluorescence intensity, and the protease products were analyzed by HPLC. IC50 values were determined from a dose-response curve, whereas Ki values were obtained by fitting initial rates to a tight-binding inhibition equation. |
Affinity data for this assay | |
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