| Assay Method Information | |
| | Caspase Inhibition Assay |
| Description: | The substrate peptides terminating in AMC/AFC are processed by caspases with or without inhibitors, and the accumulation of AMC/AFC was assessed with a Cytofluor 4000 fluorescent plate reader (Perseptive Biosystems) at excitation and emission wavelengths of 360 and 460 nm (AMC) and 395 and 530 nm (AFC), respectively. Compounds were tested at a single dose (typically 5-50 uM), and full IC50 curves were run for all compounds expressing measurable inhibitory activity. |
| Affinity data for this assay | |
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