Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  The cholinesterase assays were performed using colorimetric method reported by Ellman. Enzyme activity was determined by measuring the absorbance at 412 nm for 2 min. Each inhibitor was evaluated at several concentrations in the range from 1 nM to 20 uM. Percentage inhibition was calculated relative to a control sample, and IC50 values displayed represent the mean +/- standard deviation for triplicate assays.
Affinity data for this assay

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