Assay Method Information | |
| Enzyme Inhibition Assay |
Description: | The cholinesterase assays were performed using colorimetric method reported by Ellman. Enzyme activity was determined by measuring the absorbance at 412 nm for 2 min, relative to the drug-free control. Triplicate measurements were carried out at a total of 8 drug concentrations. The IC50 values were determined from a plot of percentage of inhibition vs -log [drug]. |
Affinity data for this assay | |
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