| Assay Method Information | |
| | Kinase Inhibition Assay |
| Description: | Briefly, the assay employed utilizes a fluorescent tracer to a given kinase. This is used to detect the addition of an anti-tag antibody. When the tracer and antibody bind to the targeted kinase, the Forster resonance energy transfer is measured. When a kinase inhibitor is added to the assay, the tracer/kinase binding will be disrupted, producing low FRET detection and indicating kinase inhibitory activity. Evaluation of kinase activity of additional kinase targets utilized the LanthaScreen binding assay or the Z′-Lyte activity assay provided by Thermo Fisher SelectScreen service. The IC50 values were determined using sequential doses, and the inhibition curves were provided by Thermo Fisher Scientific. |
| Affinity data for this assay | |
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