| Assay Method Information | |
| | Biochemical Assay for Inhibition of LpxC |
| Description: | Dilutions of test compound were pre-incubated with 5 nM P. aeruginosa or E. coli LpxC for 10 minutes at room temperature in 50 mM NaH2PO4, 500 mM sucrose, 0.2 mg/mL BSA, pH 7.2 and <1% DMSO. Reactions were initiated by the addition of 2× substrate (UDP-3-O—(R-3-hydroxydecanoyl)-N-acetylglucosamine, Carbosynth Ltd, UK, for P. aeruginosa LpxC and UDP-3-O—(R-3-hydroxymyristoyl)-N-acetylglucosamine, BOC Sciences, USA, for E. coli LpxC), in 50 mM NaH2PO4, 0.5 mg/mL BSA, pH 7.2, to a final concentration of 2.5 μM. Reactions proceeded for 1 hour at room temperature prior to quenching with and equal volume of 2% acetic acid. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |