| Assay Method Information | |
| | Activity Detection Test of DPP1 Enzyme In Vitro |
| Description: | Recombinant human DPP1 enzyme (R&D Systems, Cat. No 1071-CY) with a final concentration of 100 μg/mL and recombinant human cathepsin L (R&D System, Cat. No 952-CY) with a final concentration of 20 μg/mL were mixed, and then incubated at room temperature for 1 hour, to activate DPP1 enzyme. The activated DPP1 enzyme was diluted 100 times, and 5 μL of different concentrations of compounds and 5 μL of the diluted DPP1 enzyme were added to a 384-well plate, and incubated at room temperature for 30 minutes. After adding 10 μL of the substrate Gly-Arg-AMC (bachem, Cat. No I-1215) with a concentration of 20 M, it was continued to incubate at room temperature for 60 minutes |
| Affinity data for this assay | |
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