| Assay Method Information | |
| | KRAS G12D GTP::SOS1 Homogeneous Time-Resolved Fluorescence Binding Experiment |
| Description: | In a 384-well reaction plate (Corning, CLS4514), 0.1 microliter of the compound was added. After centrifugation, a mixture of 5 microliters of Tag2-KRAS G12D (Cisbio, 63ADKOOOCB17PEG) protein and GTP (SIGMA, V900868) with a final concentration of 10 M was added. Then, 5 microliters of Tag1-SOS1 (Cisbio, 63ADKOOOCB17PEG) protein solution was added, and the reaction was allowed to proceed at room temperature for 15 min. All protein interactions occurred in Diluent (Cisbio, 62DLBDDF) buffer solution. 10 microliters of premixed 100× Anti-Tag1-Tb and 25× Anti-Tag2-XL665 detection solutions were added, and the reaction was allowed to proceed at 4° C. for 180 min. The detection reagents occurred in Detection Buffer (Cisbio, 62DB2FDG). The reaction signals were detected by a multifunctional microplate reader, and the data was analyzed by using GraphPad Prism data analysis software. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |