| Assay Method Information | |
| | Binding to the Recombinant Mouse NLRP3 Protein |
| Description: | Monolith NT. Automated instrument was used to measure the Kd value. A range of concentrations of NSIs were incubated and His-NLRP3 pre-labeled with RED-tris-NTA dye kit (SKU: MO-L018) for 40 min in PBS-T assay buffer (1×PBS with 0.05% Tween® 20). The samples were loaded into NanoTemper® glass capillaries and series of ligand-induced changes in thermophoresis curves were gained using an Excitation power of 80% and a MST power of high. Kd values were calculated using the mass action equation with NanoTemper software from duplicate reads of an experiment. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |