Assay Method Information

Assay Name:  Biochemical Evaluation
Description:  The BTK/BTK (C481S) inhibitory activity of compound of formula A was determined in Reaction Biology Corporation, One Great Valley Parkway, Malvern, PA, USA. Full-length human BTK/BTK (C481S) enzyme and 20 μM peptide substrate [KVEKIGEGTYGVVYK] were used. The concentration of tested ATP was 10 μM. Starsporin was used as standard, of which IC50 was 3.94 nM.
Affinity data for this assay
 

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