| Assay Method Information | |
| | FLIPR Assay of Inhibitory Activity on Human TRPA1 |
| Description: | Table 3.1: The cell analysis plate was placed in an FLIPR instrument (Molecular Probes), and the compound in the compound plate was added to the corresponding hole on the cell analysis plate (First addition) via an automatic program, and the calcium ion fluorescence signal was recorded to determine whether the compound had agonistic activity. After 10 minutes, CaCl2 (Second addition) was added a final concentration of 3 mM to each well to stimulate intracellular calcium flux signals. Ca++-dependent fluorescence signal was monitored continuously at 538 nm wavelength to analyze the inhibitory activity of the compounds. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |