| Assay Method Information | |
| | Inhibition of H+/K+ ATPase Enzyme Activity Assay |
| Description: | H+/K+ ATPase Activity Experiment(1) Adding 35 μl of reaction buffer to each experimental well, and then added 35 μl of buffer 1.(2) Adding 5 μl buffer 1 containing 10% DMSO to the whole enzyme and buffer well.(3) Adding 5 μl of 10× compound working solution to the compound well and mixing well.(4) Adding 5 μl of buffer 1 to the buffer well.(5) Adding 5 μl of 10× enzyme working solution to the remaining wells, mixing and incubating at 37° C. for 30 minutes(6) Adding 5 μl of 10× ATP working solution to all experimental wells, and incubating at 37° C. for 20 min.(7) Adding 15 μl MLG chromogenic solution to all experimental wells, and uniformly mixing and incubating at room temperature for 5-30 min.(8) The reading number of 620 nm was detected by an M5 instrument. |
| Affinity data for this assay | |
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