| Assay Method Information | |
| | In-Vitro Enzymatic Activity Assay |
| Description: | 1) 10 μL of LDNA solution was added into a negative control well, 10 μL of PARP1& DNA (or PARP2& DNA) mixed solution was added into wells excluding the control well, and then 10 μL of NAD+ reagent was added into each well, and incubated at 25° C. for 60 minutes.2) The 384-well plate was washed with PBST buffer for 3 times.3) Finial concentrations of the test compounds were 1000 nM, 250 nM, 62.5 nM, 15.6 nM, 3.9 nM, 0.98 nM, 0.24 nM, 0.061 nM, 0.015 nM and 0.0038 nM. |
| Affinity data for this assay | |
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