| Assay Method Information | |
| | Inhibition of HSP47 Function |
| Description: | Fibrils were formed by adding 180 ul of phosphate-buffered saline (PBS, pH 7.4) solution to 20 ul of collagen dissolved in acidic solution (collagen solution, UK), which was measured at a wavelength of 340 nm. HSP47 (GenScript US) was added at a concentration of 9.45 μg/ml to assess HSP47 activity as the degree to which it inhibits fibril formation. Test compound was added with concentrations of 100 μM to 1 μM and the inhibitory activity of HSP47 was calculated as a percentage or EC50. |
| Affinity data for this assay | |
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