| Assay Method Information | |
| | Human C1s Enzyme Assay |
| Description: | Human complement C1s enzyme (purified from human serum, Complement Technology, Inc.) at 1.16 nM final concentration was incubated with test compound at various concentrations for 5 min at room temperature in 50 mM Tris, 1 M NaCl, pH 7.5. A synthetic substrate Z-L-Lys-SBzl and DTNB (Ellman's reagent) were added to final concentrations of 100 μM each. Absorbance at 405 nm (A405) was recorded at 30 second intervals for 30 min using a microplate spectrophotometer. IC50 values were calculated by nonlinear regression of complement C1s reaction rates as a function of test compound concentration. |
| Affinity data for this assay | |
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