| Assay Method Information | |
| | Enzyme Binding Assays (KINOMEscan) |
| Description: | PIP4K2C binding constants disclosed herein were determined using the proprietary Human PIP4K2C binding assay (Catalog number 87-0007-1094, Eurofins DiscoverX, San Diego, CA). This assay measures active site-directed competition binding to an immobilized ligand (see https://doi.org/10.1038/nbt1068, herein incorporated by reference in its entirety). Experiments were conducted using an 11-point dose response curve, in duplicate, with a top starting concentration of 5 micromolar; the Levenberg–Marquardt algorithm was used to fit a Hill equation to dose-response data to calculate IC50 values. |
| Affinity data for this assay | |
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