Assay Method Information

Assay Name:  Enzyme Inhibition Assay
Description:  HIV-1 protease inhibition was measured by a fluorescence assay using recombinant HIV-1 protease. The reactions were initiated by the addition of 1 nM HIV-1 protease and rates were determined by following the change in fluorescence intensity (excitation 340 nM, emission 490 nM) that accompanies the cleavage of the fluorogenic substrate. The percent inhibition of enzyme activity in the presence of inhibitor was determined by comparing inhibition rates with uninhibited control reactions. All IC50 values were determined with a range of inhibitor concentrations using the relation IC50 = (100/ percent inhibition - 1) x inhibitor concentration.
Affinity data for this assay
 

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