| Assay Method Information | |
| | CYP2A6 Inhibition Assay |
| Description: | To measure CYP2A6 activity, coumarin 7-hydroxylation was determined. The formation of the coumarin metabolite, 7-hydroxycoumarin, was determined fluorometrically at excitation and emissions wavelengths of 355 and 460 nm, using a Wallac Victor2 1420 counter. The amount of product formed was obtained by interpolation from a standard curve of 7-hydroxycoumarin. The IC50 values were determined using GraphPad Prism version 3.0 and are reported as an average of three experiments. Apparent Ki values were determined from the IC50 values by employing the equation of Cheng and Prusoff. |
| Affinity data for this assay | |
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