| Assay Method Information | |
| | Radiochemical Assay of PNMT Inhibitors |
| Description: | Enzyme activity is determined by measuring the amount of 3H incorporated into the substrate during the reaction. AdoMet/[methyl-3H]AdoMet serves as a methyl donor. Ki values were determined by a hyperbolic fit of the data using the single substrate-single inhibitor routine in the enzyme kinetics module(version 1.1) in SigmaPlot (version 7.0). |
| Affinity data for this assay | |
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