Assay Method Information

Assay Name:  Liquid growth assay
Description:  Saturated ACT1 wild type (WT) as well as engineered ACT1 R183K (R183K) and ACT1 R335K (R335K) cultures were adjusted to 10 OD600 and 3 uL of serial 1:3 dilutions spotted on YPD agar supplemented with indicated concentrations of Chivosazole F. Growth was scored after 3 days incubation at 30 °C.
Affinity data for this assay
 

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