| Assay Method Information | |
| | ADK Inhibition Assay |
| Description: | ADK activity was monitored by a radiochemical assay, which measures the conversion of radioactive Ado ([U-14C] adenosine or [2-3H] adenosine) to AMP in the presence of ATP and MgCl2. The radioactivity incorporated was directly proportional to the conversion rate. The reaction was terminated by aliquoting the reaction mixture onto DE-81 anion exchange filter disks. The filter disks were then dried, washed, and counted. Bound radioactivity was determined by standard scintillation spectrometry at an efficiency of 55%. |
| Affinity data for this assay | |
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