Assay Method Information
|Fluorescence Exchange Curve Assay
|The exchange curve assays were run as two half-reactions using an SLM Aminco Bowman Series 2 model SQ-340 fluorescence detector. In the first half reaction, p38 alpha MAPK and SKF 86002 are preincubated for 3 min. In the second half reaction, p38alpha MAPK is preincubated with inhibitor for 60 min. A net dissociation of the fluoroprobe is observed for the first half-reaction, and a net association is observed for the second half-reaction. The raw data from both half-reactions are fit simultaneously to an equation describing simple competitive inhibition.
|Affinity data for this assay
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