| Assay Method Information | |
| | PDE SPA Enzyme Inhibition Assay |
| Description: | PDE activity was monitored by measuring the hydrolysis of [3H]-cAMP to [3H]-AMP using scintillation proximity assay (SPA). [3H]-AMP was captured by the SPA beads, and quantified on a TopCount plate reader. Data was analyzed in Graph Pad Prism software. |
| Affinity data for this assay | |
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