Assay Method Information | |
| RAF Kinase Assay |
Description: | The biochemical activity of compound was determined by incubation with Raf kinases and substrate MEK-1 in the presence of 1-10 uM ATP/ [gamma-32P] ATP. After incubation, the reaction was stopped and the phosphorylated MEK-1 was harvested by filtration, and filter- bound radioactivity was quantified by beta-plate counter. |
Affinity data for this assay | |
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