| Assay Method Information | |
| | Scintillation Proximity Assay (SPA) |
| Description: | SPA beads conjugated to anti-mouse IgG were incubated overnight in buffer with anti-testosterone monoclonal antibody. The enzymatic reaction was monitored by its conversion of 4-androstene-3, 17-dione to testosterone. The amount of [14C] testosterone produced in each well was determined by scintillation counting on a Perkin Elmer TopCount instrument and collected as DPMs. The data was then analyzed and percent inhibition and/or IC50 values, based on 10 point serially diluted curves in duplicate or triplicate, were calculated using Microsoft Excel and XLFit programs. |
| Affinity data for this assay | |
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