| Assay Method Information | |
| | MetAP Enzyme Activity Assay |
| Description: | A coupled enzyme chromogenic assay was developed to measure methionine aminopeptidase activity by monitoring the production of free methionine with L-amino acid oxidase and horseradish peroxidase. Assays were performed in 96-well microtiter plates. The reactions were carried out at room temperature, and absorbance at 450 nm (A450) was measured every 20 s over a period of 20 min using an automatic plate reader (Molecular Devices, CA). The rate in mOD/min, calculated for each well, was converted to nM methionine releases per minute per nM MetAP in the reaction. |
| Affinity data for this assay | |
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