Assay Method Information | |
| Enzyme Inhibition Assay |
Description: | Activity was measured as a change in absorbance over time at a wavelength of 340 nm (A340), so as to monitor the disappearance of NADPH. After incubation of the enzyme with test compound and NADPH for 3 min, the reaction was initiated with the addition of DHF and the A340 was plotted over the course of 3 min. Six different concentrations of compound were utilized in the assays to determine the IC50. |
Affinity data for this assay | |
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