Assay Method Information

Assay Name:  Competitive Radioligand Displacement Assay and AR-Mediated Transcription Activation Assay
Description:  The Ki values were determined by the application of the Cheng-Prusoff equation: Ki = (IC50 x Kd)/(Kd+[L]) where [L] is the concentration of [3H]MIB (1 nM ) and Kd is the dissociation constant of the [3H]MIB determined in the saturation analysis, IC50 is determined by computer fitting data for the competitive binding of each AR ligand. EC50 is the lowest concentration of the ligand capable of maximally stimulating AR-mediated transcription during transfection experiments.
Affinity data for this assay

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