Assay Method Information

Assay Name:  Human Platelet Adhesion Assay
Description:  Flat bottom microtiter plates (96-well) were coated with soluble type I collagen. Unoccupied protein binding sites on the wells were blocked with bovine serum albumin. Human platelets were isolated from blood anticoagulated with sodium citrate by gel-filtration. The gelfiltered platelet suspension was added to the protein-coated wells in the absence or presence of an inhibitor. Following incubation for 30 min, the plates were washed, and the number of adherent platelets measured using the colorimetric assay.
Affinity data for this assay
 

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