Assay Method Information

Assay Name:  Estrogen Receptor Binding Assay.
Description:  The binding affinities were determined by a competitive radiometric binding assay using [3H]estradiol as tracer. The Kd for 3H-estradiol was determined by saturation binding to ER receptors. The IC50 values for compounds were converted to Ki using Cheng-Prusoff equation.
Affinity data for this assay
 

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