Assay Method Information

Assay Name:  Cell-Free Ligand Sensing Assay (LiSA)-LXRalpha-SRC1 Assay
Description:  The LXR LiSA measures the ligand-dependent recruitment of a 25 amino acid fragment of the steroid receptor coactivator 1 (SRC1) to the ligand-binding domain of the receptor. The biotinylated protein was incubated with streptavidin-AlloPhycoCyanin, biotinylated SRC-1 peptide, and streptavidin-labeled europium. The fluorescence resonance energy transfer was then counted on a Wallac Victor fluorescent plate reader in a time-resolved mode. The relative fluorescence was measured at 665 nm.
Affinity data for this assay

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