Assay Method Information

Assay Name:  GPR40 FDSS Assay
Description:   Human embryonic kidney (HEK293) cells expressing human GPR40 receptors were grown in Dulbecco modified Eagles medium (DMEM)/F12 media supplemented with 10% fetal FBS and glutamine. Changes in ligand-induced calcium-dependent intracellular fluorescence were measured with a fluorometric plate reader (FDSS; Hamamatsu Corp., Bridgewater, NJ).
Affinity data for this assay
 

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