| Assay Method Information | |
| | Enzymatic Inhibition Assay |
| Description: | Phospholipase A2 was assayed using unlabeled lipids as substrate and 1-anilinonaphthalene-8-sulfonate (ANS) as a probe for the interfacial reaction. The assay was carried out in a 96-well plate utilizing a multiwell fluorometer (SpectraMax GeminiXS, Molecular Devices). The reaction was monitored by excitation at 377 nm and emission at 470 nm. Fluorescent signals were monitored by a kinetics mode program. The IC50 values were determined by plotting concentration-velocity curves from at least three separate experiments. The initial velocities of the change in fluorescence intensity with various inhibitor concentrations were used to calculate the IC50 values. |
| Affinity data for this assay | |
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