Assay Method Information | |
| Inhibition of Eg5 ATPase Activity |
Description: | The microtubule-activated ATPase rates were measured using the pyruvate kinase/lactate dehydrogenase-linked assay. To optimize the signal for basal Eg5 activity at low protein concentration, measurements were performed in the presence of 300 mM NaCl. Eg5 at seven different concentrations was incubated at room temperature for 25 min with test compounds from 0 to 5 uM. After the reaction, the resulting decrease in absorbance at 340 nm was measured using the 96-well Sunrise photometer. |
Affinity data for this assay | |
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