| Assay Method Information | |
| | IP Receptor Filter Binding Assay and cAMP Assay |
| Description: | IP receptor binding activity was quantified via a filter binding assay measuring displacement of [3H]-Iloprost binding to human platelet membranes. Raw data were fit to the Michaelis-Menten equation to calculate IC50 values. EC50/IC50 values were obtained from functional antagonism assay by measuring the inhibition of cAMP production induced by Iloprost in human erythroleukemia (HEL) cells using a Perkin-Elmer LANCETM cAMP detection kit. |
| Affinity data for this assay | |
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