Assay Method Information | |
| Microtubule-Stimulated KSP ATPase Activity Assay |
Description: | KSP ATPase activity was determined by measuring production of ADP (ADPQuest Assay, DiscoverX). Raw fluorescence data were normalized to negative (DMSO) and positive (compound 2a, as a racemic mixture) controls. Data analysis was performed using Spotfire (v8.1, Spotfire, Inc.) and Kalypsys software. |
Affinity data for this assay | |
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