| Assay Method Information | |
| | Determination of PI3K Activity |
| Description: | The luminescence-based kinase assay with recombinant phosphatidylinositol 3-kinase (Upstate Cell Signaling Solutions) was used for the determination of IC50 values. Luminosity was measured using an Ultra 384 instrument (Teca). IC50 values were calculated from the luminescence-concentration curves using the nonlinear curve fitting capabilities of Prism (Version4, GraphPad software). |
| Affinity data for this assay | |
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