| Assay Method Information | |
| | 3H-CGP 12177 Whole Cell Binding Assay |
| Description: | The whole cell-binding studies were undertaken in CHO cell lines stably expressing each beta-adrenoceptor subtype. Nonspecific binding was determined in the presence of 100 nM CGP 20712A for the beta1-adrenoceptor, 100 nM ICI 118551 for the beta2-adrenoceptor, and 100 uM CGP 12177 for the beta3-adrenoceptor. Increasing concentrations of the competing ligand were used until the specific binding of 3H-CGP 12177 was completely inhibited. IC50 was determined as the concentration required to inhibit 50% of the specific binding. From the IC50 value and the known concentration of radioligand 3H-CGP 12177, a Kd (concentration at which half the receptors are bound by the competing ligand) value was calculated using a equation. |
| Affinity data for this assay | |
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