| Assay Method Information | |
| | Fluorescence Polarization Assay |
| Description: | Fluorescence polarization was measured on an Ultra plate reader at excitation and emission wavelengths of 485 and 530 nm, respectively. The equilibrium binding curves were drawn by plotting polarization units as a function of recombinant protein concentration. The Smac mimetics were evaluated for their ability to displace the fluorescent probe from the protein. The Ki values were derived from IC50s according to Cheng-Prusoff equation. |
| Affinity data for this assay | |
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