| Assay Method Information | |
| | Enzyme Inhibition Assay |
| Description: | Enzyme activity assays were performed by monitoring the rate of enzyme-dependent NADPH oxidation at an absorbance of 340 nm over several minutes. All enzyme assays were performed with a single, limiting concentration of enzyme and saturating concentrations of NADPH and dihydrofolate. IC50 values were calculated as the average of three independent experiments. |
| Affinity data for this assay | |
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