Assay Method Information

Assay Name:  Reverse Transcriptase Assay
Description:  The inhibition of the RNA-dependent DNA polymerase activity of the HIV-1 reverse transcriptase enzyme was measured using a primer extension assay. The substrate-incorporated radioactivity was measured using the Topcount NXT with a reading time of 1 minute/well and IC50 were deduced from the dose response curves obtained.
Affinity data for this assay
 

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