Assay Method Information

Assay Name:  DNA Strand-Transfer Activity Assay
Description:  In the strand-transfer assay, the biotinylated donor DNA was bound to streptavidin-coated plates, and integrase was added to each well to allow processing of the donor DNA. Serially diluted compounds and DIG-tagged target DNA were then added to each well, and 3-end joining reaction was allowed to proceed for 30 min. Signal was detected using horseradishperoxidase-conjugated anti-Digoxin antibody through chemiluminescence measurement.
Affinity data for this assay

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