| Assay Method Information | |
| | DGAT Enzyme Inhibition Assay |
| Description: | The assay mixture contains test compound, membrane protein, and 1,2 dioleoyl-sn-glycerol in a 96-deep well plate. The reaction was started by adding substrate [14C]oleoyl coenzyme A and incubated. After the reaction was stopped, radioactive triolein product was separated into the organic heptane phase. DGAT activity was quantified by counting aliquots of the upper heptane layer by liquid scintillography. |
| Affinity data for this assay | |
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