| Assay Method Information | |
| | Cell-Based Transcription Assay |
| Description: | The HepG2 cells were co-transfected with DNA construct containing PPAR-Gal4 chimeric receptor and Gal4-luciferase reporter. The luciferase activity was corrected for transfection efficiency using the beta-galactosidase activity as an internal standard. EC50 is the concentration of test compounds needed to induce 50% of the maximum luciferase activity. All transfection experiments were repeated at least three times. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |