Assay Method Information

Assay Name:  PPAR alpha Fluorescence Polarization Assay (IC50) and Cell-Based Transcription Assay (EC50)
Description:  For hPPAR alpha, percentage inhibition was calculated relative to unlabeled GW2331, which was used as the active site-specific competitive binder. Fluorescence polarization measurements were performed on an LJL Analyst (Molecular Devices, Sunnyvale, CA) using an excitation wavelength of 485 nm and an emission wavelength of 530 nm. IC50, Hill slope, and maximal and minimal inhibition values were calculated in XLfit (IDBS, Guildford, Surrey, UK). EC50 is the concentration of test compounds needed to induce 50% of the maximum luciferase activity in HEK cells transfected with PPAR and Gal4-luciferase reporter construct.
Affinity data for this assay

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