Assay Method Information

Assay Name:  Scintillation Proximity Assay (IC50) and Cell-Based Transactivation Assay (EC50)
Description:  Binding affinity (IC50) was evaluated using scintillation proximity assay with radiolabeled ligand and biotinylated receptor. Because the scintillant is contained within the SPA bead, only radiolabel that is attached to the bead is detected. The receptor-ligand complex is bound to the bead through interaction between the biotinylated receptor and the streptavidin moiety located on the surface of the bead. EC50 was measured by luciferase transcriptional reporter gene assays. Efficacies are relative to GW4064.
Affinity data for this assay
 

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