Assay Method Information

Assay Name:  WNV Protease Inhibition Assay
Description:  Inhibitors were assayed in a 96-well plate format using enzyme conjugate WNV CF40-gly-NS3pro187, which was preincubated with various concentrations of test compounds at 37 deg C for 30 min. The reaction was then initiated by the addition of substrate Bz-nKRR-AMC. Reaction progress was monitored continuously by following the increase in fluorescence (excitation 385 nm, emission 465 nm) on a TECAN Safire plate reader. IC50 values were derived for inhibitors by fitting the calculated initial velocities to a nonlinear regression curve fit using GraphPad Prism software (San Diego, CA). Each point of the IC50 curve was carried out in duplicate during a single experiment.
Affinity data for this assay

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