| Assay Method Information | |
| | WNV Protease Inhibition Assay |
| Description: | Inhibitors were assayed in a 96-well plate format using enzyme conjugate WNV CF40-gly-NS3pro187, which was preincubated with various concentrations of test compounds at 37 deg C for 30 min. The reaction was then initiated by the addition of substrate Bz-nKRR-AMC. Reaction progress was monitored continuously by following the increase in fluorescence (excitation 385 nm, emission 465 nm) on a TECAN Safire plate reader. IC50 values were derived for inhibitors by fitting the calculated initial velocities to a nonlinear regression curve fit using GraphPad Prism software (San Diego, CA). Each point of the IC50 curve was carried out in duplicate during a single experiment. |
| Affinity data for this assay | |
|---|---|
| If you find an error in this entry please send us an E-mail | |