| Assay Method Information | |
| | Enzyme Assays and Inhibition Study |
| Description: | The inhibitory activities of MAO-A and MAO-B were assessed using kynuramine (0.06 mM) and benzylamine (0.3 mM) as substrates, respectively, following a standardized protocol[1]. Absorbance readings were taken using a previously described method[2]. The selectivity index (SI) of MAO-B was determined by the formula: (IC50 of MAO-A)/(IC50 of MAO-B). Test compounds were compared against standard compounds toloxatone and clorgyline for MAO-A inhibition and safinamide and pargyline for MAO-B inhibition. IC50 values of lead compounds were measured at six different concentrations, while other effective compounds were assessed at three concentrations. |
| Affinity data for this assay | |
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